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econo pac chromatography columns  (Bio-Rad)
96
Bio-Rad econo pac chromatography columns
Econo Pac Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromatography column  (Bio-Rad)
96
Bio-Rad chromatography column
Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cationexchanger  (Bio-Rad)
99
Bio-Rad cationexchanger
Cationexchanger, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econo pac chromatography column  (Bio-Rad)
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Bio-Rad econo pac chromatography column
Econo Pac Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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size exclusion chromatography sec  (Bio-Rad)
96
Bio-Rad size exclusion chromatography sec
The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion <t>chromatography</t> <t>(SEC),</t> where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.
Size Exclusion Chromatography Sec, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glass econo-column  (Bio-Rad)
95
Bio-Rad glass econo-column
The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion <t>chromatography</t> <t>(SEC),</t> where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.
Glass Econo Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cation exchange chromatography  (Bio-Rad)
94
Bio-Rad cation exchange chromatography
The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion <t>chromatography</t> <t>(SEC),</t> where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.
Cation Exchange Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econo pac protein a chromatography columns  (Bio-Rad)
96
Bio-Rad econo pac protein a chromatography columns
The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion <t>chromatography</t> <t>(SEC),</t> where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.
Econo Pac Protein A Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel chromatography column  (Bio-Rad)
96
Bio-Rad gel chromatography column
The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion <t>chromatography</t> <t>(SEC),</t> where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.
Gel Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion chromatography (SEC), where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.

Journal: Frontiers in Plant Science

Article Title: Systematic characterization of extracellular vesicles from potato ( Solanum tuberosum cv. Laura) roots and peels: biophysical properties and proteomic profiling

doi: 10.3389/fpls.2024.1477614

Figure Lengend Snippet: The experimental design used for the collection, isolation, and characterization of extracellular vesicles released by potato (cv. Laura) roots and peels. Independent experiments were carried out to collect initial sampling solutions containing root exudates of potato ( Solanum tuberosum , cultivar: Laura) plants grown hydroponically and apoplastic washes collected from potato peels of the same cultivar by a vacuum infiltration method using a vesicle isolation buffer. Three replicates were conducted for each experiment. The purification process was a combination of differential centrifugation steps and size exclusion chromatography (SEC), where 20 purified fractions (500 µL each) of the samples were collected for further examination. Biophysical and chemical analyses were conducted on these extracellular vesicles (EVs) using the Bradford protein assay, nanoparticle tracking analysis (NTA), and transmission electron microscopy (TEM). The EV proteome of the samples was evaluated using mass spectrometry where purified EV samples (n = 3) and crude samples (n = 3) from each potato peel and root sample were used for comparison.

Article Snippet: EVs were isolated from concentrated sample solutions using size exclusion chromatography (SEC) (Econo-pac ® Disposable chromatography column, Bio-Rad, Berkeley, CA, USA) packed with a 10-cm column of cross-linked 4% agarose matrix with 90-μm beads (Sepharose 4 fast flowTM, GE HealthCare Bio-Sciences AB, Uppsala, Sweden) and using Dulbecco’s phosphate-buffered saline (DPBS; Sigma-Aldrich, St. Louis, MO, USA) as the equilibration buffer.

Techniques: Isolation, Sampling, Purification, Centrifugation, Size-exclusion Chromatography, Bradford Protein Assay, Transmission Assay, Electron Microscopy, Mass Spectrometry, Comparison

The particle and protein distribution for fractions collected from Solanum tuberosum (cv. Laura) peel apoplastic fluid (A) and root growth medium (B) using size exclusion chromatography (SEC). Fractions 4–8 and 5–8 contain the highest number of particles (vesicles) with a minimal amount of protein contamination for peel and root samples, respectively. n = 3; error bars represent the standard error of the mean ( ± SEM). Particle number was measured using the scatter mode of Nanoparticle Tracking Analysis (NTA), and the total protein concentration was measured using the Bradford protein assay.

Journal: Frontiers in Plant Science

Article Title: Systematic characterization of extracellular vesicles from potato ( Solanum tuberosum cv. Laura) roots and peels: biophysical properties and proteomic profiling

doi: 10.3389/fpls.2024.1477614

Figure Lengend Snippet: The particle and protein distribution for fractions collected from Solanum tuberosum (cv. Laura) peel apoplastic fluid (A) and root growth medium (B) using size exclusion chromatography (SEC). Fractions 4–8 and 5–8 contain the highest number of particles (vesicles) with a minimal amount of protein contamination for peel and root samples, respectively. n = 3; error bars represent the standard error of the mean ( ± SEM). Particle number was measured using the scatter mode of Nanoparticle Tracking Analysis (NTA), and the total protein concentration was measured using the Bradford protein assay.

Article Snippet: EVs were isolated from concentrated sample solutions using size exclusion chromatography (SEC) (Econo-pac ® Disposable chromatography column, Bio-Rad, Berkeley, CA, USA) packed with a 10-cm column of cross-linked 4% agarose matrix with 90-μm beads (Sepharose 4 fast flowTM, GE HealthCare Bio-Sciences AB, Uppsala, Sweden) and using Dulbecco’s phosphate-buffered saline (DPBS; Sigma-Aldrich, St. Louis, MO, USA) as the equilibration buffer.

Techniques: Size-exclusion Chromatography, Protein Concentration, Bradford Protein Assay